The bacteriology of the female reproductive organs of cattle and its relation to the diseases of calves .. by Carpenter Charles Milton 1895-

Author:Carpenter, Charles Milton, 1895- [from old catalog]
Language: eng
Format: epub, pdf
Tags: Generative organs, Cattle, Veterinary bacteriology
Publisher: [Albany, J. B. Lyon company, printers
Published: 1922-03-25T05:00:00+00:00

In many eases only pairs were observed hut this was probably due to the medium and to tlie length of time the culture was grown after isolation. Quite a large number of hemolytic streptococci belonging to the Beta type have been recovered, as well as a few strains belonging to the group that Brown (37) calls " alpha prime." In the latter case immediately surrounding the colony is a narrow zone of corpuscles that have not become hemolyzed, while surrounding this zone is a broader one in which hemolysis of the corpuscles has taken place. The various results of the inoculations of these streptococci from the genital canal made upon the sugar media have not yielded anything definite as yet. There seem to be many slight variations in each strain which make anything like classification at present difficult.

The staphylococci have been mainly ^staphylococcus alhns and Staphylococcus aureus. Some of them have been hemolytic and some non-hemolytic. A larger percentage of the cultures of Staphylococcus aureus has been hemolytic than of Staphylococcus albns.

Iiu.cteriuni abortuui was recovered from many of the different genital organs. Before definitely designating an organism Bacterium ahortutn, I have injected it into guinea pigs to see if it produced the characteristic lesions, and have made antigens from the cultures and checked them against positive abortion serum. The other biological and staining characteristics have also been carefully worked out.

A few Gram positive spore-bearing rods and Gram negative rods which could not be identified were isolated at various times, especially from the cervi.x and anterior part of the vagina, as recorded on the charts. Occasionally organisms from the colon-aerogenes group, paracolon types, Ps. pyocya-veus and B. pyogenes liave been recovered but their ciiltural characters are too well known to need description.

Experimental

In connection with this investigation, experimental work has been carried on with calves of various ages and with a limited number of heifers. The purpose of these experiments has been to find: First, what the effect of some of these organisms would be upon the reproductive organs of young animals if bacteria were injected directly into the uterine cavity; second, whether the genital canal could free itself of infection when bacteria were injected directly into the uterine cavity, and, if it could do this, how long it would take for the microorganisms to disappear; third, whether these bacteria had any affinity for, or would localize in, the genital canal, if suspensions of the organisms were injected intravenously.

In case of the males the different suspensions of bacteria were injected into the scrotal sac or testicular tissue with a hypodermic syringe. The uteri of the females were exposed through the flank after laparotomy had been performed, and the bacterial suspensions injected with a hypodermic syringe directly into the uterine cavity. As a rule the udders of the calves were injected with the same organism which was placed in the uteri. Intravenous injections were made in the usual manner.

Very heavy bacterial suspensions of Bacterium abortum were used, about twice as cloudy as a bouillon culture of }{.

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